Abstract : Objective To investigate the effects of non-uniform constant magnetic field N and S poles on learning and memory in mice. Methods and avoidance experiment method locomotor activity were observed in non-uniform constant magnetic field N, S pole influence on learning and memory; light microscope after applying constant magnetic field a non-uniform N, S poles treatment, brain neuronal cell morphology change. Experimental results show that avoidance N pole magnetic field group and blank control group, the number of errors achievement test (mis2take, M value) decreases, the value of M to reduce the memory test, there were significant differences (P <0. 05); S-pole magnetic field group compared with control group, the value of M to reduce the memory test, there was a significant difference (P <0. 05); measured in each group had no significant difference (P locomotor activity in mice> 005.); each of the groups of brain The structure of the Yuan has not changed significantly. Conclusion Non prophylactic treatment of a uniform constant magnetic field, can improve learning and memory in mice, and at the very N.
Keywords : learning and memory ; non-uniform constant magnetic field ; autonomous activity
Magnetic therapy is widely used in clinical practice, the role of the nervous system mainly in terms of analgesia and sedation, but whether it will affect learning and memory have not been reported. I avoidance test in mice Method methods and locomotor activity were observed in non-uniform constant magnetic field N, S pole and memory ability in learning and mice, and non-uniform light microscope constant magnetic field treatment, the cerebral cortex of mice histological changes in the structure of neurons, are reported below.
1 Materials and methods
1. 1 experimental animals and grouping
54 Kunming mice, male and female, body weight (20 ± 2) g (provided by the Experimental Animal Center of Medical Bethune). They were randomly divided into 3 groups , namely blank control group, magnetic field N- pole group and magnetic field S- pole group , with 18 groups in each group .
1. 2 magnetic field devices and instruments
1cm × 1 cm × 0. 2 cm of N and S pole plates each with 24 pieces of the same polarity , each set of front, rear, left and right spacing
3 cm, arranged in four rows, N or S poles face mouse body, an outer fixed to the bottom of the box two hard plastic rats, measuring the pole plate and the adjacent pole plate electrode free horizontal spacing, vertical spacing, and the center point of the corresponding diagonal rat magnetic field strength inside the bottom box, at the N pole group are 30.0, 3.0, 3.1 and 1. 4 mT; group is in the S pole 46.0, 3.9, 4.0 and 1, respectively. 4 mT . The mice in the magnetic field N and S pole groups were housed in a mouse box with the N pole facing the mouse body or the S pole facing the mouse body. Mouse avoidance test automatic recorder, mouse locomotor activity program to automatically control device, are manufactured Institute of Materia Medica.
1. 3 methods
Mouse darkness test : The animals were tested after 3 days of adaptation to the environment , room temperature 15 °C ~ 18 °C. After 15 days of magnetic field treatment , the mice were trained to avoid darkness experiments. The method according to literature mice were placed in the light chamber of the passive avoidance test instrument, first enter the dark chamber mice were recorded within 5 min being the time required for the shock latency (the EL), and the mice were recorded within 5 min electric shock The number of times as the number of errors (M) . After 24 h, the darkening experiment was repeated , and the shocked M and EL were recorded as indicators for evaluating the memory function of the mice.
Determination of locomotor activity in mice: Mice were placed in the locomotor activity boxes automatically recorded, recording the number of activities within 5 min.
Light microscopy specimen preparation and statistical analysis: measured in learning and memory function, in each group were sacrificed immediately decapitation 6, the fixed portion of the cerebral hemispheres same front frontal cortex, a conventional light microscope method, were performed to observe . The changes in the histological structure of brain neurons in each group were compared. Results as mean ± standard deviation (x ± s), said plurality of samples using analysis of variance and q are numbers between pairwise comparison test.
2 results
The effects of non-uniform constant magnetic field on learning, memory and autonomic activity in mice are shown in Tables 1 to 2 . Light microscope observation, no significant changes between the groups of brain neurons histology.
3 Discussion
Experiments have shown that the constant magnetic field has the biological effects of the nervous system , but the mechanism of action is not very clear.
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