Standard operating procedure for pipette sterilization

In fact, not long ago, Lebe told everyone that there is a difference between the disinfection and sterilization of pipettes. Today we refrain from sterilizing, which refers to the killing or removal of all microorganisms on objects, including the highly resistant bacterial spores. When you use a pipette, you sometimes get a sucking solution to suck the solution into the pipette. At this time, everyone is thinking about whether the pipette can be taken apart and cleaned. (Before the pipette was discovered by Lebe) It is necessary to recalibrate and sterilize the pipette after cleaning. At this time, the general pipette can not be autoclaved, and it can only be surface sterilized by ultraviolet lamp, and the process can be as long as 15 minutes. With the development of the times, there are now half-sterilizable pipettes ( Eppendorf ) and whole sterilizable pipettes (such as IKA 's ALPHA series and PRECISION series). These pipettes can be sterilized. Part can be autoclaved at 121 ° C, 1 bar, 20 minutes. However, everyone should pay attention to the fact that the half-sterilizable pipette must be removed from the non-sterile before it is sterilized. Otherwise, the whole pipette will be reimbursed, so Lebe thinks that it is possible to buy one. The pipette of the bacteria is more convenient.

Ika pipette

In addition to the above two sterilization methods, Lebe also recommended a wiping method - sterilizing the pipette with 75% alcohol.

I just saw a friend on the forum saying that I bought a whole sterilized pipette until the pipette was scrapped. I didn’t sterilize it. In fact, Lebe wants to say that there is really no place to sterilize? Or you didn’t follow it. The state has to do it. . . . . . Others said that sterilization needs to be disassembled, and it is very troublesome to recalibrate after each disassembly. Then Lebe asked you if your lab does not need to be checked during the period? Pipettes are measuring instruments that are themselves subject to irregular calibration to ensure the accuracy of the experimental data.

Natural Pigment

Natural pigments are food pigments obtained from natural resources. Pigments extracted mainly from animal and plant tissues and microorganisms (cultures), in which vegetative colorants predominate. Natural pigments not only have the function of coloring food, but also have physiological activity. At present, there are 48 kinds of edible natural pigments approved to be used in China, commonly used chili red, beet red, monascus red, cochineal red, gaolianghong, sodium copper chlorophyllin, turmeric, gardenia yellow, carotene, algal blue pigment, cocoa pigment, caramel pigment and so on.

Roselle Calyx Extract,Phycocyanin,Lutein,Butterfly Pea Flower Powder,Beta Carotene Powder,Sodium Copper Chlorophyllin

Xi'an Gawen Biotechnology Co., Ltd , https://www.amulyn-bio.com