General: GC9800 Gas Chromatograph Manufacturer: Jinan Fine Electronic Technology Co., Ltd.
General: LC01 Liquid Chromatograph Manufacturer: Jinan Fine Electronic Technology Co., Ltd.
Due to the diversity of sample analysis, many times we often don't know the difference between high-performance liquid chromatography and gas chromatography. How can we quickly understand the difference between the two? Here, the gas chromatograph manufacturer Jinan Precision Electronic Technology Co., Ltd. engineers to popularize this knowledge for everyone.
The difference between gas chromatography and liquid chromatography:
High performance liquid chromatography: a sample or organic medium solution suitable for solution after dissolution, independent of the volatility and thermal stability of the sample. More than 70% of the organic compounds can be analyzed by high performance liquid chromatography. In particular, separation analysis of compounds with high boiling point, macromolecules, poor volatility, strong polarity, and poor thermal stability shows advantages.
Gas Chromatography: Adapt to sample analysis that is easy to vaporize, has good thermal stability, and has a relatively low boiling point. However, it is not suitable for sample analysis of high boiling point, poor volatility, poor thermal stability, ionic and high polymer; the disadvantage of chromatography is that it is difficult to characterize the unknown, if there is no known pure sample or known pure sample The chromatogram of a chromatogram makes it difficult to determine what a particular peak represents.
Samples that can be directly separated by a gas chromatograph are volatile and thermally stable, and generally have a boiling point of no more than 500 °C. According to relevant statistics, 20% to 25% of the currently known compounds can be directly analyzed by gas chromatography, and the rest can be analyzed by liquid chromatography in principle. That is to say, the gas chromatograph is far more analytical than the liquid chromatograph. It should be noted that some samples that cannot be directly analyzed by gas chromatography can be indirectly analyzed by gas chromatography by special injection techniques such as headspace injection and lysis injection. This is the case, for example, in pyrolysis chromatography of polymeric materials. This has expanded the range of objects analyzed by gas chromatography to some extent. In addition, gas chromatography is more suitable for analysis of permanent gases than liquid chromatography.
Gas chromatography has the advantages of good separation ability, high sensitivity, fast analysis speed and convenient operation. However, due to technical conditions, substances with too high boiling point or substances with poor thermal stability are difficult to analyze by gas chromatography. Generally, a derivatization method or a cleavage method may be employed for a substance which is less volatile or is easily decomposed by heat at 500 ° C or lower. High performance liquid chromatography requires only a sample to be made into a solution without the need for gasification and is therefore not limited by the volatility of the sample. For organic substances with high boiling point, poor thermal stability and relatively high molecular weight (more than 400 or more) (some substances account for almost 75% to 80% of the total organic matter), high performance liquid chromatography can be used for separation and analysis in principle. According to statistics, among the known compounds, about 20% can be analyzed by gas chromatography, and about 70 to 80% can be analyzed by liquid chromatography.
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