The concept of the cell lines and cell lines in the high school biology textbooks of the People's Education Edition (the one approved in 2002) is different from or even completely opposite to the concept of the Zhejiang Science Edition. The two concepts of the "Modern Biotechnology Special Topics" high school textbook approved by the People's Education Edition in 2004 did not appear. According to the data, the two concepts were mixed and the concept was unclear.
The concept of human-educated high school biological cell strain emphasizes the cell population that can be subcultured and can be subcultured, and does not emphasize the purity of the cell population; the concept of the cell strain of Zheke Edition emphasizes the purity of the cell population, and the cell strain is considered to be the result of cloning. .
The concept of the human-educated high school biological cell line emphasizes that some of the genetic changes in these cells have occurred, with cancerous cells that can be subcultured; the Zyco version of the cell line concept emphasizes that the cell line is the first cell population after successful passage.
The definition of human-educated high school biological cell lines and cell lines is distinguished by passage algebra, and whether the genetic material changes as a marker; while the two concepts in the Zhe Ke version are independent of the passage algebra, the genetic material is not changed. . The cell line emphasizes the unity of the cell components in the cell culture, and all the genetic and biochemical characteristics are identical; the cell line does not emphasize the purity, but there should be a certain type of cells occupying the vast majority.
According to many recent materials, the description of the Zhejiang version should be relatively scientific. With the development of science and technology, the concept has also evolved and improved. The animal cell culture technology has developed rapidly, and many concepts are believed to be standardized.
Cell strain ( Cell Strain )
A culture having a specific property or marker obtained from a primary culture or a cell line by a selection method or a clone formation method is called a cell strain, that is, the cell strain is isolated or cultured by single cells or by screening. A population of cells formed by single cell proliferation. The specific nature or signature of the cell line must be present throughout the culture period.
Cell line
Primary cultures are cell lines after successful first passage, so cell lines can be broadly referred to as cells that are likely to be passaged. If the passage cannot be continued, or the number of passages is limited, it can be called a finite cell line. If it can be continuously cultured, it is called a continuous cell line. It can be cultured for more than 50 generations and cultured indefinitely.
According to P384, the principle of biological reaction engineering edited by Cao Zhuan, the chromosomes in the cells of the infinite cell line often become aneuploid and become transformed cells. Transformed cells have unlimited growth ability, are easier to grow in culture, and are widely used in the production of biological products.
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