Spring grass carp is good for vaccination

Grass carp is favored by consumers because of its delicate meat, large individuals, and less intermuscular pricks. However, the “four diseases” (red bark, gill disease, enteritis, and hemorrhagic disease) of grass carp restrict the development of grass carp farming. Long-term production practice proves that grass carp immunization technology is currently an effective way to solve the "four diseases" of grass carp.

First, principle

At low temperature (8°C-20°C), the metabolic capacity of grass carp is relatively weak. The second-in-age grass carp fingerlings can be given a new dose of immunization antigen through the injection route, and after 5-30 days of low temperature (10°C-15°C) Induction, so that the injected grass carp fish species can complete the initial immunity, and the immune resistance level of grass carp can be rapidly strengthened when the water temperature is raised to above 20°C, so as to achieve the goal of reducing the chance of the second-in-age grass carp.

Second, the method

1. Time choice. It is selected in spring when the water temperature is 8°C to 22°C. The northern regions try to select the lower temperature of the water in March, which is beneficial to the prevention of saprophytic disease. Diseased grass carp should not be immunized.

2. Fish species preparation. All kinds of robust and disease-free grass carp fingerlings with a size of 8.33 cm or more can be immunized. Disinfect fish fingerlings with 0.5% saline solution before injection. Control time is 5-10 minutes. In order to facilitate the production operation, the fish species can be anesthetized with an aqueous solution of trichlorfon.

3. Tool selection. Syringes, needles, and dilution vessels must be sterilized with 75% alcohol or boiled with boiling water. For fish species below 9.99 cm, 4.5# or 5# injection needles are generally used. For fish species above 11.66 cm, 5.5# or 6# injection needles are generally used. For fish species above 16.65cm, 7# injection needles are generally used. . In order to prevent the injection needle from being deeply injured during the injection and the internal organs of the fish, a small plastic tube can be placed on the injection needle, and the exposed tip length is slightly longer than the thickness of the abdominal muscle of the fish.

4. Vaccine dilution. The concentrated liquid is diluted 10 times with 0.65% physiological saline before use. A bottle of diluted vaccine is used up once as much as possible. If it is used up once, the required amount can be used with a syringe, and the needle eye is sealed with a medical tape after extraction.

5. Injection site and dose. At the base of the pelvic fins, the needle pierces the fish body at 45 degrees. For each species of fingerling less than 9.99 cm in size, inject 0.5 ml of diluted vaccine into each tail. Sizes of 9.99 to 16.65 cm fingerlings should be injected with 0.5 to 1 ml of diluted vaccine on each tail, and fingerlings of 16.65 cm in size must be injected on each tail. 1 ml of diluted vaccine.

Third, the cost

Under normal circumstances, the cost of injecting vaccine for each grass carp species is about 2 cents. Calculated by putting 1,000 grasses per acre, the vaccine cost is only about 20 yuan per mu, and the comprehensive economic benefits are very significant.

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