Recently, Chen Shaoliang Laboratory of the College of Biological Science and Technology of Beijing Forestry University used the research results of “non-invasive micro-testing technology†in the publication “Plant, Cell & Environment†(2008 IF 4.666) . This achievement combines non-injury micro-measurement technology with laser confocal and X-ray microanalysis techniques to study the signaling pathways involved in the regulation of K + /Na + dynamic balance in Populus euphratica under salt stress, and found that plasma membrane H + The coupled transporter (PM H + -coupled transport system) can stimulate H 2 O 2 and cytosolic Ca 2+ signaling, and then regulate K + /Na + dynamics by regulating K + channels and Na + /H + antiporters. balance. The non-invasive micro-test technique is an indispensable tool for obtaining ion-transmembrane flow information in this study.
In the past year or more, Chen Shaoliang has published three high-level research papers using non-invasive micro-test technology, reaching or exceeding the level of scientific research in the same field abroad, and ranking among the world's leading laboratories. The laboratory has accumulated rich experience in applying non-invasive micro-testing technology, and has trained a group of proficient non-invasive micro-testing techniques represented by the first author of this article, Sun Jian (also the first author of the previous two research papers). Excellent students, I believe that a lot of high-quality results will be born soon.
Introduction to Professor Chen Shaoliang, College of Biological Science and Technology, Beijing Forestry University
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ABSTRACT:
Using confocal microscopy, X-ray microanalysis and the scanning ion-selective electrode technique, we investigated the signalling of H 2 O 2 , cytosolic Ca 2+ ([Ca2+]cyt) and the
PM H + -coupled transport system in K + /Na + homeostasis control in NaCl-stressed calluses of Populus euphratica.An obvious Na + /H + antiport was seen in salinized cells;however, NaCl stress caused a net K + efflux, because H 2 O 2 levels, regulated upwards by salinity, contributed to ionic homeostasis,because H 2 O 2 restrictions by DPI or DMTU treated enhanced K + efflux and decreased Na + /H + antiport activity. Induced a net Ca 2+ influx and a subsequent rise of [Ca 2+ ]cyt, which is involved in H2O2-mediated K + /Na + homeostasis in salinized P. euphratica cells. When callus cells were pretreated with inhibitors of the Na + /H + antiport system, the NaCl-induced elevation of H 2 O 2 and [Ca 2+ ]cyt was correspondingly restricted, leading to a greater K+ efflux and a more pronounced reduction in Na + /H + antiport activity. Results suggest that The PM H + -coupled transport system mediates H + translocation and triggers the stress Signalling of H 2 O 2 and Ca 2+ , which results in a K + /Na + homeostasis via mediations of K + channels and the Na + /H + antiport system in the PM of NaCl-stressed cells. Signalling pathway of P. euphratica cells is proposed.
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