In China, research on deer artificial insemination began in 53 years, two years earlier than abroad. In 2005, 52 sika deer were inseminated in 3 units in Jilin Province, and the rate of conception was 163. The Harbin Institute of Special Products has successfully developed fine tube frozen sperm for red deer, and produced more than 3,000 pieces in five years. According to statistics from 22 production units in the province, there are 30 red deer for artificial insemination. Artificial insemination can improve the utilization rate of the excellent species of the stag. Using the artificial insemination method, each superior species of the male deer collects more than 20 times in one breeding period, and can infuse more than 500 female deer. Artificial insemination can be scientifically selected for breeding. There are plans to use good breeding deer to maintain the health of breeding deer and reduce the top rack and casualties among species of male deer. Artificial insemination can overcome the boundaries of regions and countries, expand the area of ​​application of improved varieties, and at the same time reduce the spread of diseases and successfully complete the breeding and breeding of deer of different species and strain types. Frozen sperm can be stored for a long period of time and transported easily without being limited by the life of the buck. Artificial insemination technology: The commonly used methods of sperm sampling are electrostimulation and sperm extraction methods, which are the most widely used, with a success rate of 20% or more. Pseudo-vagina extraction is the most ideal method for sperm extraction. The electrical stimulation of the spermatozoa requires the use of an electrical stimulation seizure device, and the electrical stimulation of the seizure device includes an electrostimulator and a rectal probe. The main technical parameters of the electrical stimulator: The power supply voltage is 220V. The output adjustable voltage is 220V. The output current is 35mA. Rectal scoop: using a rigid plastic tube, the diameter of which differs depending on the type of deer, on which three fixed, mutually insulated metal rings are fitted, which are led by two wires, two poles, and the pins and the output socket of the stimulator. coupling. The sika deer rectal probe has a total length of 36 cm, a diameter of 562 cm, a distance of 26 cm between two metal rings, and 3 metal rings. Electrostimulation Semen Collection Method: Semi-anaesthetized with xylolthiazole thiazolyl, with a dose of 51 micrograms and 1 kilogram body weight. When the deer is in a semi-conscious state, the deer is laid down in the posture and direction that has been prepared, or it is secured with a security device. deer. After the deer was decoctioned, immediately enema with soapy water, drain the stool in the rectum, wash the foreskin, squeeze out the effusion, cut the surrounding hair and rinse, ready to collect sperm. Slowly insert the rectal probe from the anus, insert 22cm into the deer, adjust the stimulator, select the frequency, switch on the power supply, and start the voltage again and again. Each time the power is on for 1 second, the interval is 1 second, the voltage rises gradually, and the stimulation is enhanced. The sika deer stimulating frequency is appropriate, the power is six times, and the stimulating voltage reaches the deer penis after three times of electrifying, the sperm of the deer begins to be refined. At this time, care should be taken to collect and pay attention to heat preservation and darkness. The collection bottle temperature is close to the deer body temperature 22C. Afterwards, add diluted solution or keep frozen. Pay attention to the quiet environment during anesthesia so as not to be disturbed. After the injection of anesthetic drugs, do not catch the deer prematurely, wait until the anesthesia is good, and then make another artificial protection to prevent over-struggling damage to the deer. Resting quietly after harvesting prevents injuries. According to Zhao Shizhen’s statistics on the 532 electric stimulations of 32 plum stags, the success rate was 2363, and the average ejaculation volume was 52 ml. Electrical stimulation collected sika deer quality test results: semen color milky white or light white, micro-sputum, ejaculation volume 53 ml, semen 125 ml, sperm density 52.3 billion / ml, malformation rate of 52%. Semen liquid nitrogen cryopreservation. Frozen particle dilution: 3 ml of the base fluid, 3 ml of egg yolk, 6 ml of glycerol, 50,000 each of cyanine and streptomycin, and 5.3-fold dilution. Twenty-three milliliters of sodium citrate, thaw in cold water. Sika deer ejaculation volume 5.3 ml, diluted by 2.5 times, generally frozen before rejuvenation after the reactivation, sperm density is medium, medium and dense. Semen cryopreservation: The diluted semen is placed in a small sterilized glass or small test tube without any gaps. The plug is tightly closed to remove the air from the vial. The vial containing the semen is then filled with water. Inside the beaker, store it in the refrigerator. The cooling process of cryopreservation of semen requires 3.3 hours, otherwise it cannot inhibit sperm metabolism and exercise. Cannot be lower than 1C, otherwise sperm will die. Save in the refrigerator, should pay attention to emergency measures taken when power outages. Liquid nitrogen tanks for frozen semen can be used for long-distance transportation. Before the fresh essence is transported, it shall be divided into small bottles, filled with tight plugs, and sealed with external tape. May be transported in short distances and can be carried away in the outer pocket. Ice can be used in February, cold water for cooling, keep below 3C, available thermos or double plastic bags. The vials are wrapped in gauze to prevent temperature changes and impacts, and the outer layer is filled with cold water. Insemination can be used intrauterine insemination, through the endoscope, the semen directly into the uterine horn, due to technical relations, the country has not yet carried out. Cervical insemination is the estrous female standing in a specific Baoding cabinet Baoding or anesthesia with Baoninning Baoding, opened the female deer vagina with a open device, with the vas deferens semen, aligned at the cervix and into the 5.3 cm fine. The deer rectal grasping insemination method: After the red deer is insured, the inseminator wears latex gloves and the outer wall is soaked with soapy water. The cervix is ​​held in the rectum and the other hand holds the insemination gun. The vagina is inserted to align the cervix. Insert 5.3 cm of insemination inward. The insemination period was better after two consecutive inseminations within 3 hours of estrus; the insemination rate was 60% higher after 2 hours of estrus. China Agricultural Network Editor
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