Biochemical incubators are widely used in many fields. Below we will introduce the multi-tube fermentation method and membrane method of the living incubator.
Multi-tube fermentation
Initial fermentation experiment
The fermentation tube of the biochemical incubator is filled with a single or triple lactose peptone culture solution, and a small glass tube is inverted inside. Each sample was inoculated with three different water samples. The same inoculum water sample should have five tubes. The water samples were inoculated separately into the biochemical incubator fermentation tube containing lactose peptone culture solution at 37 °C ± 0.5 °C. Culture for 24h ± 2h. Lactose can play a role in selection because many bacteria cannot ferment lactose, while coliforms can ferment lactose and produce acid to produce gas. Bromocresol purple is added to the culture solution as an acidity indicator. After the bacteria produce acid, the culture solution turns from purple to yellow. The fermentation tube producing acid-producing gas indicates that the test is positive. If the gas is not obvious in the inverted tube, the fermentation tube can be tapped, and small bubbles rise to be positive.
2. Filter method
The biochemical incubator membrane is a microporous membrane with a pore size of 0.45 μm. The water sample is injected into the sterilized filter with the filter membrane. After suction filtration, the bacteria are trapped on the membrane, and then the membrane is attached to the M-FC medium and cultured at 44.5 ° C ± 0.5 ° C. 24 h ± 2 h. The fecal coliform colonies were blue or blue-green on M-FC medium, and other non-fecal coliform colonies were gray, yellowish or colorless. Normally, non-fecal coliform colonies are rarely seen on M-FC medium due to temperature and selective action of the rose salt reagent.
Double fermentation experiment
The biochemical incubator fermentation tube with slight shaking of the initial fermentation test results was transferred to the EC medium with a 3 mm inoculating loop, and cultured at 44.5 ° C ± 0.5 ° C for 24 h ± 2 h (increased culture temperature) Can cause growth of coliforms that are not conducive to the natural environment). Immediately after the culture, the fermentation tube produced gas was confirmed to be positive for fecal coliforms.
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