Overview:
This kit is suitable for PCR detection of various animal, plant and viral DNA. Users can be based on the analyzed genes,
With a pair of primers, the amplification can be done according to the standard system provided in the manual. The kit is equipped with conventional primers and templates.
Can be used as a control experiment.
Composition: (Original number: A026)
PER013-1 PER013-2
Concentration 20T 50T
1. Solution A (dNTPs) 10mM 15μl 30μl
2. Solution B (Taq enzyme) 2u/μl 15μl 30μl
3. Solution C (10×PCR buffer) 10×60μl 150μl
4. Solution D (template) 20ng 10μl 10μl
5. Solution E (primer) 20pmol 10μl 10μl
6. Solution F (ddH2O) 0.5ml 1.5ml
PCR reaction:
1. Prepare 25 ul PCR reaction according to the following table:
Composition quantity
Solution A (dNTPs) 0.5μl
Solution B (Taq enzyme) 0.5μl
Solution C (10×PCR buffer) 2.5μl
Solution D (template) 1.0μl
Solution E (primer) 1.0μl
Solution F (ddH2O) with water to make up to 25μl
2. Mix and centrifuge for a few seconds, amplify on a PCR instrument, and observe the results on agarose gel electrophoresis.
3. The recommended amplification parameters for the control experiments are as follows:
Pre-denaturation at 94 ° C for 2 minutes
(94°C denaturation for 45 seconds)
55°C renaturation 45 seconds
72°C extension for 60 seconds) Amplification 30 rounds
72 ° C extension plus 5 minutes
Store at 4 ° C
Description:
1. Users can adjust the amplification system and parameters according to their actual needs.
2. The kit provides primers for amplification of the target fragment size of 1 kb.
3. Storage: Store at -20 °C for at least 10 months.
This kit is suitable for PCR detection of various animal, plant and viral DNA. Users can be based on the analyzed genes,
With a pair of primers, the amplification can be done according to the standard system provided in the manual. The kit is equipped with conventional primers and templates.
Can be used as a control experiment.
Composition: (Original number: A026)
PER013-1 PER013-2
Concentration 20T 50T
1. Solution A (dNTPs) 10mM 15μl 30μl
2. Solution B (Taq enzyme) 2u/μl 15μl 30μl
3. Solution C (10×PCR buffer) 10×60μl 150μl
4. Solution D (template) 20ng 10μl 10μl
5. Solution E (primer) 20pmol 10μl 10μl
6. Solution F (ddH2O) 0.5ml 1.5ml
PCR reaction:
1. Prepare 25 ul PCR reaction according to the following table:
Composition quantity
Solution A (dNTPs) 0.5μl
Solution B (Taq enzyme) 0.5μl
Solution C (10×PCR buffer) 2.5μl
Solution D (template) 1.0μl
Solution E (primer) 1.0μl
Solution F (ddH2O) with water to make up to 25μl
2. Mix and centrifuge for a few seconds, amplify on a PCR instrument, and observe the results on agarose gel electrophoresis.
3. The recommended amplification parameters for the control experiments are as follows:
Pre-denaturation at 94 ° C for 2 minutes
(94°C denaturation for 45 seconds)
55°C renaturation 45 seconds
72°C extension for 60 seconds) Amplification 30 rounds
72 ° C extension plus 5 minutes
Store at 4 ° C
Description:
1. Users can adjust the amplification system and parameters according to their actual needs.
2. The kit provides primers for amplification of the target fragment size of 1 kb.
3. Storage: Store at -20 °C for at least 10 months.
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