- Experimental principle (the development history of water maze, as well as simple experimental principles and application fields, as well as the development in China and major manufacturers at home and abroad)
- The composition of the Morris water maze (mainly divided into hardware and image acquisition, software)
- Hardware guidelines (hardware guidelines mentioned in domestic and foreign literature, we mainly refer to domestic literature)
- Experimental process guidelines (experimental details)
- Statistical methodological criteria (following statistical principles)
- Guidelines for evaluating indicators (interpretation of experimental data)
- Experimental Scope Guidelines (Experiments apply to four areas, with 10 advantages of the water maze)
First, the experimental principle
In the early 1980s, British psychologist Morris and his colleagues used rats to search for targets in opaque pools containing water and milk mixtures to study the damage of the hippocampus and other brain regions of rats. The memory and spatial orientation and cognitive abilities have achieved remarkable results. The device is not only novel in concept, reasonable in experimental design, but also simple and practical in method. It is also convenient to observe and record the time, strategy and swimming trajectory of animals hidden in the underwater platform after entering the water, so that the animals can be analyzed and inferred. The ability to learn, remember and spatially recognize. Although the initial experimental subjects were rats, the labyrinth system has since become a classical procedure for assessing spatial learning and memory in rodents, and is widely used in basic and applied research in the fields of neurobiology and pharmacology. It can objectively measure changes in animal spatial memory, working memory, and spatial discrimination. Therefore, this research method quickly attracted the attention of neuroscientists in various countries, and this method is called the Morris water maze method. There are many foreign companies that produce Morris water maze, such as TSE in Germany and Sandiegoinstruments in the United States. In the late 1980s, the Institute of Psychology of the Chinese Academy of Sciences established the first Morris water maze laboratory in China. At the beginning of the year, the Morris water maze image automatic acquisition and processing system was established. There have been many Morris water maze manufacturers in the country, such as Shanghai Xinsoft, Beijing Pharmaceutical Research Institute, etc., but from international to domestic, Morris water maze hardware equipment, software systems, experimental methods, and processing methods of experimental data. They are all different. Therefore, from the invention of the Morris water maze method to the present, it has been more than 20 years old, and a set of relatively standard experimental criteria should be developed.
Second, the composition of the Morris water maze
The Morris experimental system consists of a water maze device, an automatic collection of water maze images, and a software analysis system.
1. Morris water maze device: mainly consists of a water-filled pool and a platform with adjustable height and movable position.
2, water maze image automatic acquisition and software analysis system: the main components of the system are cameras, computers and image capture cards. This system has a variety of models, but the working principle and process are roughly similar: the camera captures the rat swimming image (analog signal) and inputs it to the image acquisition card in the computer for analog/digital conversion. The simulated image of the rat swimming is converted into digital image. Stored in the hard disk, image analysis of digital images to get the relevant test parameters. The software analysis system can automatically collect parameters such as the water entry position of the animal, the speed of swimming, the time required to search for the target, the running track and the search strategy, and can collect and analyze various collected data.
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