Application of Small Animal Micro CT Experimental Platform in the Study of Mouse Ankle Arthritis

Experimental purposes: Human factors induced arthritis in mice, leading to severe bone defects and bone loss in the fore paw ankle joint. The mice with arthritis are then treated with drugs to find and compare the therapeutic effects of different drugs.

Experimental method: The experiment was carried out on the small animal Micro-CT public experimental platform of Guangzhou Zhongke Yusheng Company. The experiment was divided into three groups. The first group was a CT scan of the forepaw ankle joint of normal mice. The second group was a CT scan of the forepaw of arthritic mice. The third group was the mouse fore paw of drug intervention. Perform a CT scan. Comparisons were made from parameters such as morphological structure and bone density.

Experimental results: There were significant differences in the structural morphology and bone density data of the forearm and ankle joints between arthritis and normal mice.

Guangzhou Zhongke Yusheng established the first micro-computer experimental platform for small animals in China in 2009. In the past few years, it has provided a large number of professional services for major medical colleges, hospitals and research institutions in China. I got a very good reputation. Zhongke Yusheng's self-developed small animal Micor CT system contains 17 bone parameters. For example, bone density BMD, tissue mineral density TMD, number of trabecular bone, trabecular thickness, and trabecular separation. It can be seamlessly interfaced with other third-party software (such as Mimics, Ansys, etc.) to meet your various needs.

Now share some data pictures of the experiment with peer researchers or relevant study researchers, I hope to help you in related fields.

The scanning parameters of this experiment Micro-CT are voltage 50Kvp, power 40W, 6 frames superimposed, and field of view 4cm×4 cm. Three sets of mouse forearm ankle joint samples were selected for the experimental materials, and scanned after fixation. Three-dimensional image capture and bone parameter analysis were performed after scan reconstruction.

In the above figure, we obtained a three-dimensional view of the ankle joint by Micro-CT scanning. We can clearly distinguish the difference in the structural morphology of the forepaw between normal and arthritic mice. The density of the ankle bone in normal mice is similar to that of the surrounding bone, and the surface of the cortical bone is smooth, and the joint bone structure is neat and regular. In comparison, the bone mass of the ankle joint of the arthritic mice was low, the surface of the cortical bone was rough, the joint bone structure was irregular, and there was a significant loss of bone dissolution. Further comparison of bone mineral density BMD, tissue mineral density TMD and other parameters, found that the BMD and TMD values ​​of the ankle bone parameters of normal mice were about 3 times higher than those of the joint mice. In the last picture, we can find that the morphological structure of the ankle joint of the drug interference group is similar to that of the normal group. In the bone parameter analysis, the values ​​of BMD and TMD were between the normal group and the disease. This illustrates the role of drugs in the active treatment of arthritis in mice.

The main application direction of Micro-CT is orthopedic research. Micro-CT has an unshakable position in the study of fine bone morphology, bone density and trabecular parameters. Many valuable high-end bone research papers at home and abroad are obtained through the Micro-CT platform. Zhongke Yusheng is a technology company that is supported by the Institute of Automation of the Chinese Academy of Sciences and Xi'an University of Electronic Science and Technology. It is the only technology company engaged in small animal CT imaging research and conducting external experimental service platforms. Academic papers based on the Micro-CT of Zhongke Yusheng have been published in the world's top medical journals and journals. With the deepening of bone science research, Micro-CT will certainly be further promoted and applied. If you have research needs in this area, you can log on to our company's homepage http:// to learn about the latest developments in our products and services.