Application of ImageXpress® Micro Confocal High Content Imaging System in 3D Cell Culture

Establishing physiologically relevant in vitro models is critical to further understanding the mechanisms of neurological diseases and targeted drug development. iPSC- derived neurons show great promise for compound screening and disease modeling, however, the use of three-dimensional (3D) cultures as an effective method for the development of assays for neuronal cells has been developed. 3D cell culture is thought to be closer to human tissue, including structures, cell tissues, cell-cells, and cell-matrix interactions.

The 3D cell culture mode can more realistically reflect the process of cell interaction and physiological changes during compound screening than the traditional monolayer cell culture mode. Manual analysis of the corresponding detection analysis can be complicated and cumbersome, and becomes more and more difficult as the sample size increases. The automated system enables 3D cell imaging analysis in a more precise and throughput-based manner, and the ImageXpress® Micro Confocal Confocal Imaging System will be your competent research assistant.

Currently, the more common of the two ball 3D cell culture model comprises a hydrogel and 3DProSeed ^TM microfluidic OrganoPlate® internet culture system. Hydrogels help cell migration by incorporating MMP cleavage sites and contain RGD cell adhesion motifs to support cell adhesion. This hydrogel prefabricated plate is easy to use and is well compatible with automation equipment (Figure 1). OrganoPlate® is a high-throughput platform that combines the latest 3D cell culture technology, PhaseguidesTM and microfluidic technology. It contains 96 tissue microarrays suitable for long-term culture of live cells, suitable for screening purposes, and with standard laboratory equipment or Automated systems are compatible, such as the ImageXpress® Micro Confocal Confocal High Content Imaging Analysis System (Figure 2).