Dr. Pan Qingjie of Animal Science and Technology College of Laiyang Agricultural College and other scientific and technical personnel participated in the national 863 "mammary gland bioreactor" major project --- transgenic animal research has achieved initial results, the research group produced transgenic rabbits, June 2004 Later in the Laiyang Agricultural Science Research Base Dongying Tianyi Biological Technology Co., Ltd. was born in succession, 184 rabbits. The test results showed that there were 89 positive individuals with a positive rate of 48.4%, and new breakthroughs were made in the transgene efficiency and the number of transgenic animals. Dr. Pan Qingjie, from Institute of Animal Science and Technology, Laiyang Agricultural College, Shandong Province, and other scientific and technical personnel participated in the national 863 "mammary gland bioreactor" major special subject-transgenic animal research has achieved initial results, the title group produced transgenic rabbits in 2004 6 On the 10th of the month, a 184 rabbits were born in the breeding farm of Tianyi Biotechnology Co., Ltd. of Dongying City, a scientific research base of Laiyang Agricultural College. According to recent tests, there were 89 positive individuals with a positive rate of 48.4%, and new breakthroughs have been made in the transgene efficiency and the number of transgenic animals. Animal mammary gland bioreactors are pharmaceutical proteins or other biologically active substances for the treatment and health care of human diseases by producing transgenic animals and using their mammary glands for large-scale production. Mammary glands of transgenic animals can replace bacteria and cells and other organisms for fermentation, and can effectively post-process and modify the produced protein. Therefore, animal mammary gland bioreactors are considered to be one of the most promising directions in the current research on transgenic animals, and they are also extremely attractive industries in genetic engineering pharmaceuticals. At present, among the various technical methods for preparing transgenic animals, most of the techniques require expensive equipment and skilled technicians in the process of operation, so the large investment and long cycle time limit the operability of transgenic animal production. In this case, the research group of Laiyang Agricultural College designed different research routes and systematically studied the application of techniques such as somatic gene targeting, BAC vector method, and sperm carrier method in transgenic animals, and achieved certain research results. The research on sperm carrier method has made very valuable progress and reached the international advanced level.