NO regulates Cd2+ influx during Cd2+-induced programmed cell death in tobacco BY-2 cells

NO is an important biologically active gas and functional molecule. NO acts as a signaling molecule when plants are subjected to biotic and abiotic stresses, including heavy metal Cd 2+ . Cd 2+ is a non-essential and toxic heavy metal that causes programmed cell death (PCD) in plants.

Chinese scientists used non-invasive micro-test techniques to study the regulatory factors of PCD in tobacco BY-2 cells exposed to 150 μM CdCl 2 . It was found that Cd 2+ induced PCD was accompanied by a significant increase in NO content. The application of the NO release agent SNP can accelerate the PCD and also promote the influx of Cd 2+ , resulting in more accumulation of intracellular Cd 2+ . NO synthetase inhibitor L-NAME and NO-specific scavenger cPTIO alleviated the toxicity caused by Cd 2+ , decreased the rate of Cd 2+ entering cells, reduced the accumulation of intracellular Cd 2+ , and even led to Cd 2+ outflow. . Based on these findings, that by adjusting the NO absorption Cd 2+ and Cd 2+ promote accumulation BY-2 cells resulted in the induction of PCD Cd 2+ plays a positive role in regulation.

Direct determination of Cd 2+ and out of this study NMT technology, the first time to obtain evidence of NO transport regulation Cd 2+, Cd 2+ regulation mechanism of absorption is very important for the understanding of plants, but also according to this The study looked for plants enriched in Cd 2+ to help with phytoremediation.

Key words: tobacco BY-2 cells, Cd 2+ , NO, programmed cell death (PCD), non-invasive micro-test technique

References: Ma W, et al . Planta , 2010, 232: 325 - 335.

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Top panel: Effect of NO on Cd 2+ flow rate in tobacco BY-2 cells after treatment with 150 μm CdCl 2 for 6 h .

 

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