1. Natural seeding method The seed of Phalaenopsis that has been sent out from cracked pods is sown in the pots of the parent plants. This is due to the symbiotic bacteria necessary for the germination of Phalaenopsis seeds in the plant material of the parent plants. The natural sowing method is simple and easy, without the need for complicated aseptic procedures and operating tools. It is suitable for the general family of phalaenopsis growers. However, this method has very little chance of success and is rarely used.
2. The method of aseptic seeding is to wash the unopened mature pods first, then immerse them in 75%-90% ethanol or chloroform for 2 to 3 seconds, and then use 5% to 10% bleach solution or 3%. Hydrogen peroxide soaked for 5 to 20 minutes. The seeds are removed and soaked in the same sterilized water for 5-20 minutes. The solution is then removed by filtration, the seeds are removed, and the seeds are evenly spread on the surface of the prepared medium in the bottle with a fine needle. The culture conditions were light intensity 2000 to 3000 lux, 10 to 18 hours per day, and the temperature was maintained at 20 to 26°C. After 9 to 10 months, the young seedlings grow 2 to 3 leaves and can be planted in pots. This method is a highly scientific work and is usually conducted in an organization training laboratory or in a large-scale, strictly managed tissue culture factory.
Second, peduncle buds propagation method Many species of phalaenopsis after the flowers withered, the pedicel internode can often grow rooted seedlings, cut the next planting will be able to grow into a new Phalaenopsis. Artificial priming can be used to ensure that the pedicels of Phalaenopsis grow pedicels for reproduction. The method is to first cut off the part of the pedicel that has been flowered, and then carefully remove the bracts between the first to third sections of the pedicel with a blade or sharp knife to expose the buds in the internodes; Hormone or stilbene and other hormones are evenly applied to the exposed internodes; after treatment, the blue plants are placed at the half shade, and the temperature is maintained at 25-28°C. After 2 to 3 weeks, the buds grow out of the leaves. After 3 months, it became a Phalaenopsis seedling with 3 to 4 leaves with aerial roots; cutting off the seedlings to form a new orchid plant.
Third, the broken heart germination breeding flange strain due to frost damage, pests, diseases and human factors and other factors caused by the destruction of its growing point, after a period of time, from the orchid stems near the base of the stem section grow 1-2 sprouts . You can use this feature to breed Phalaenopsis. The specific method of operation is: to remove the highest heart of the stem, pay attention to the destruction of the growth point of the shoot, so that it can not grow upward; the wound is dried or disinfected and sterilized with a bactericide, after a period of time can be near Two to three new shoots grow on the base of the stem; when the new shoots grow and a root system emerges from the base, it can be cut and planted separately to become a new plant.
Fourth, cut stem propagation method Cut stem propagation principle is to destroy the shoot tip growth point, in order to induce latent bud growth. Phyllostachys pubescens plants have latent buds at 1 to 3, but they do not germinate. After the cultivars continue to grow upward and the stem sections are longer, the upper parts of the plants with roots are cut off with sterilized sharps or scissors, and the new pots are implanted to make them continue to grow. The lower part with roots is given proper water management. Soon sprouts 1 to 3 sprouts (depending on the character of the plant itself and its management method). If the stem length of the plant is longer, multiple segments may also be considered. As long as each segment has 2 to 3 nodes or 2 to 3 cm in length and more than one root, it is possible to grow into a new plant, but if the plant All of the roots and stems have died of dead, this method is invalid.
Fifth, tissue culture method The use of tissue culture method to propagate Phalaenopsis can obtain the same excellent genetic characteristics as the mother plant. Phalaenopsis seedlings produced by this method are known as seedlings or tissue seedlings. The plant tissues (explants) used for the meristem cultivation may be apical buds (shoot tips), stem segments (dormant buds), or young leaves or root tips, but the most common one is the use of a butterfly orchid. Pedicel. The use of pedicels as explants not only does not damage the plants, but also induces them easily. Older peduncles or flowering pedicels are mainly pedicellial buds, while young pedicels, in addition to peduncle buds, can also be used as a culture material.
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