Cyanine dyes are typically synthesized from 2, 3, 5 or 7 methine structures with reactive groups on either or both sides of the nitrogen side chain such that they can be chemically linked to nucleic acid or protein molecules. Labeling is done for visualization and quantification purposes. Biological applications include comparative genomic hybridization and gene chips for transcriptomics, as well as various studies in proteomics, such as RNA localization, molecular interaction studies by fluorescence energy transfer (FRET) and fluorescent immunoassays. Cy3 is a fluorescent dye commonly used in fluorescence microscopy applications and immunohistochemistry. Cy3 is greenish yellow (~548 nm excitation, ~565 nm emission). Due to the inherent high extinction coefficient, this dye is also easily detected by the naked eye on the gel and in solution. Labeled antibodies can be used in a variety of techniques, such as flow cytometry.
Why recommend Abbkine's LinKineTM Cy3 Coupling Kit?
To see how effective the fluorescent dye Cy3 coupling kit is, you need to understand the characteristics of the Cy3 coupling kit, and Abbkine's LinKineTM Cy3 coupling kit can be used directly to couple antibodies or other proteins. Moreover, the novel Cy3 fluorescent labeling dye is one of the latest fluorescent labels with high fluorescence intensity, low background and strong quenching resistance. The Cy3 coupling kit includes a purification column to ensure rapid and efficient removal of unreacted dyes and excellent protein recovery.
Product Name Product No. Product Details
LinKineTM Cy3 Coupling Kit KTL0220 Details
LinKineTM Cy3 Coupling Kit Features and Benefits:
1. Optimized solution - follow standard experimental procedures with excellent pre-activated dyes, protein ratios, and proprietary solution formulations for optimal activity or fluorescence.
2. Easy to operate - three steps can be achieved by binding the coupling group to the primary amine site of the antibody or other protein.
3. Customization - changing the molar ratio, reaction buffer and pH, and other parameters, can achieve different levels of coupling and activity.
Links: EliKineTM ELISA Quantitation Kit Family
More validated immunological related kits.
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