The pruning period of grapes in winter is 2 to 3 weeks after the plants are naturally deciduous and before the bleeding, usually in December. In general, the germination rate and seed setting rate at the base of the parent branch of grapes are lower, while the middle and upper bud eyes are higher. For varieties with vigorous growth and low fruiting female shoots, medium and long shoots are mainly pruned; the flower buds at the lower part of weak branches are better differentiated, and short shoots are the main pruning method. For the pruning of Kyoho, Hongdi, and Qiuli grapes, the pruning method is mainly mid-top pruning, with a mixture of long and short shoots. For the pruning of the fruiting mother branches, 4 to 8 buds are generally left when using mid-shoot pruning; when using short-shoot pruning, 2 to 3 buds are left, and when long-shoot pruning, 8-12 buds are left. For specific pruning, leave branches with full buds and tight scales, dense tissues, short internodes, large internodes protruding, deep purple in color and fully mature branches without pests and diseases. As for the number of pruning parent branches, it can be judged according to the comprehensive factors such as variety characteristics, shelf characteristics, tree age, and yield. Generally, 8 to 10 fruiting parent branches are reserved per square meter on the shelf surface of the fence, and 6 to 8 fruiting parent branches per square meter on the shelf surface.
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The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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