Abbkine offers a full range of closed serums, everything

What is Blocking Serum = Normal Serum?

The closed serum is a blood-stained non-immune normal animal, then no anticoagulant, blood coagulation, blood clot contraction, and a pale yellow liquid. The serum after special treatment is especially suitable for blocking treatment in various immunological experiments, such as IHC, IF/ICC, ELISA, etc.

What is the difference between serum and plasma?

Plasma refers to the pale yellow or colorless translucent liquid remaining in the blood after removal of cellular components, and generally requires anticoagulation treatment. Serum differs from plasma in that serum does not contain certain coagulation factors such as fibrinogen that are consumed during coagulation, adding chemicals released by vascular endothelial cells and platelets during blood coagulation.

Why do you need to be closed?

The blocking step reduces the background signal produced by non-specific binding of the primary and secondary antibodies to non-target sites. Ideally, the blocking agent will occupy a "sticky" site in the sample, such as an exposed charged or hydrophobic protein surface, without interfering with the primary/secondary antibody recognition target epitope, thereby maximizing the signal-to-noise ratio. (S/N).

Is it good to use closed serum?

The blocked serum is suitable for the blocking of samples in immunohistochemistry or immunofluorescence experiments. The non-specific binding site of the protein in the sample to be tested can be blocked, and the endogenous Fc fragment binding site in the sample can be blocked to block the binding of the antibody to the Fc receptor in the sample, thereby reducing the background and reducing false positives. . For experiments with polyclonal antibodies, normal serum from a secondary resistant species is the gold standard for blocking treatment.

Abbkine offers you a full range of closed serums

Why would you recommend the closed serum of the Abbkine brand?

As a professional product in the field of life sciences, Aijiejie Technology always adheres to the principle of not only pushing the right, not pushing expensive, providing you with cost-effective products and accelerating scientific research results. Abbkine's blocked serum has the following advantages:

1. High quality: Normal serum is blood collected from an unimmunized normal adult host, processed by lipid extraction and dialysis. The use of patented technology in blood collection and serum extraction greatly avoids hemolysis.
2. Wide range of uses: IHC, ICC, IF, ELISA.
3. Applicable and convenient: liquid form product (stock solution), no need to dissolve, can be used directly diluted with PBS.
4. Stable quality: If stored at -20 °C, it can usually be put for 2-3 years.
5. Excellent formula: Compared with other imported brands, it has doubled the preservative content and has less toxicity/impact on cells and proteins.
6. Species: Provides a full range of closed serum to meet the needs of most customers.
7. Off-the-shelf: A large amount of stock reserves to ensure the timeliness of delivery.
8. Cost-effective: affordable high-quality products (affinity price, international quality) [30-40% lower than imported brands; lower than some domestic brands]

How to use closed serum?

It is recommended to dilute the blocking serum solution 5-20 times with PBS, prepare 5%-20% working solution, directly add on tissue sections or cell smears, incubate at 37 °C for 10-30 minutes, remove serum, do not wash Add the appropriate dilution of the primary antibody working solution, incubate at 37 ° C for 1-2 h or 4 ° C overnight.

Practical Tips

1. The optimal dilution ratio needs to be determined by the experimenter. The recommended initial dilution ratios are as follows: IHC-p (1:10-20); IF / ICC (1:10-20); ELISA (1:100).
2. The blocking serum cannot contain the target protein, and unlike the primary antibody source, the blocked serum of the same source as the secondary antibody can be used.
3. After the end of the closure, the serum was removed by filter paper, and the primary antibody was directly added without washing.
4. The amount required for each slice can be calculated by referring to 50 ul PBS + 5 ul serum.
5. How to store and thaw serum without compromising product quality:

After the serum was taken out of the freezer, it was first placed in a refrigerator at 2 to 8 ° C to be melted, and then completely melted at room temperature. However, it must be noted that the melting process must be regularly shaken evenly. When stored at 2-8 ° C for a long time, various proteins and lipoproteins in the serum may aggregate to form a precipitate or visible turbidity. Therefore, it is recommended to store serum below -20 °C and avoid repeated freezing and thawing. If the lid is not used after opening, we recommend storing it at 4 ° C, but not more than one month.

For more validated immunology related products, please consult:

Abbkine China General Agent Website:

Or a local authorized Abbkine agent.

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