With the continuous expansion of the mushroom cultivation area in the northeast, mushroom species are also updated year by year. The method of tissue separation is a method of asexual reproduction of shiitake mushrooms. This method of separating mother plants is easy to operate and has a small variation in the progeny of reproduction. It can maintain the excellent characteristics of the original strains. Therefore, most scientific research institutions and seed production units are inspected in varieties. Afterwards, this method was adopted to maintain the excellent seed quality of the strains, and the original parent species was used to propagate the original species and cultivated species, and applied to production. After 3 to 4 years, the strains gradually began to show signs of aging.
1. The choice of separation with mushrooms in the selection of excellent varieties of mushroom single mushroom weight, round caps, stipe short, thick flesh, fresh colors, there are scales and no pests single mushroom, require open degrees in the 7-8 points are in the normal growth of mushroom.
2. Disinfection of mushrooms The selected mushrooms were brought into the inoculation room, and the mushroom roots were cut off. The stipe was clamped with sterile tweezers and wiped with 1% of mercury-mercuring water or 75% of medical alcohol for 2 to 3 times. Then, the separation tool is sterilized by a conventional method and transferred to a sterile separation zone.
3. The tissue pieces of the isolated mushroom were disinfected with alcohol under the aseptic condition, and then the mushrooms were split vertically by hand. The inoculated knife was quickly used to infuse the mushroom cap and the stipe at the junction of the mushroom. Grab two blades horizontally and cut a knife every 2 mm in the middle of the kerf. Use a pair of tweezers to pick up the tissue block. Quickly insert the middle part of the slant medium and press it slightly to ensure that the tissue mass and the medium are sufficient. contact.
4. Cultivation of mother seedlings After inoculation, the test tubes are placed in an incubator at a temperature of 23 to 25°C. After 2 to 3 days, there are gray and white radial hyphae around the tissue blocks, and hyphae begin to spread after 1 to 2 days. Test tube slope. About 12 to 15 days, the mycelium can fill the whole test tube, and the test tube, which is pure white, stout, and uniform, is laid on the slant and remains as the parent species, and the rest are eliminated. After the mother plant is revived at low temperature, it can expand the original species, cultivated species and carry out fruiting experiments.
1. The choice of separation with mushrooms in the selection of excellent varieties of mushroom single mushroom weight, round caps, stipe short, thick flesh, fresh colors, there are scales and no pests single mushroom, require open degrees in the 7-8 points are in the normal growth of mushroom.
2. Disinfection of mushrooms The selected mushrooms were brought into the inoculation room, and the mushroom roots were cut off. The stipe was clamped with sterile tweezers and wiped with 1% of mercury-mercuring water or 75% of medical alcohol for 2 to 3 times. Then, the separation tool is sterilized by a conventional method and transferred to a sterile separation zone.
3. The tissue pieces of the isolated mushroom were disinfected with alcohol under the aseptic condition, and then the mushrooms were split vertically by hand. The inoculated knife was quickly used to infuse the mushroom cap and the stipe at the junction of the mushroom. Grab two blades horizontally and cut a knife every 2 mm in the middle of the kerf. Use a pair of tweezers to pick up the tissue block. Quickly insert the middle part of the slant medium and press it slightly to ensure that the tissue mass and the medium are sufficient. contact.
4. Cultivation of mother seedlings After inoculation, the test tubes are placed in an incubator at a temperature of 23 to 25°C. After 2 to 3 days, there are gray and white radial hyphae around the tissue blocks, and hyphae begin to spread after 1 to 2 days. Test tube slope. About 12 to 15 days, the mycelium can fill the whole test tube, and the test tube, which is pure white, stout, and uniform, is laid on the slant and remains as the parent species, and the rest are eliminated. After the mother plant is revived at low temperature, it can expand the original species, cultivated species and carry out fruiting experiments.
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